SOLVED:The number of molecules of inorganic phosphate incorporated into organic form per atom of oxygen consumed, termed the P: O ratio, was frequently used as an index of oxidative phosphorylation. (a) What is the relation of the P: O ratio to the ratio of the number of protons translocated per electron pair (H^ / 2 e^-) and the ratio of the number of protons needed to synthesize ATP and transport it to the cytoplasm (P / H^ ) ? (b) What are the P: O ratios for electrons donated by matrix NADH
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SOLVED:Enzymes that bind phosphoribosyl-5-phosphate (PRPP) have a common structural fold, the PRT fold, which unites them as a structural family. PRT here refers to the phosphoribosyl transferase activity displayed by some family members. Typically, in such reactions, PPi is displaced from PRPP by a nitrogen-containing nucleophile. Several such reactions occur in purine metabolism. Identify two such reactions where the enzyme involved is likely to be a PRT family member.
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