Direct fibroblast reprogramming: an emerging strategy for treating ...
Feb 27, 2025 · Innovative methods for fibroblast reprogramming like small molecules, CRISPRa, modified mRNA, and the challenges of cellular heterogeneity and senescence faced by in vivo direct …
Chemical reprogramming of fibroblasts into retinal pigment ... - Nature
Dec 9, 2025 · This study presents a two-step chemical reprogramming strategy that converts fibroblasts into functional retinal pigment epithelium cells, offering a safe and scalable alternative for vision ...
Efficient reprogramming of human fibroblasts using RNA reprogramming …
Dec 1, 2022 · In summary, RNA reprogramming with DAPT/iDOT1L can accelerate reprogramming of human fibroblast cells without decreasing p53 expression, and we showed that this protocol is an …
(PDF) Direct fibroblast reprogramming: an emerging strategy for ...
Feb 27, 2025 · Fibroblasts are frequently employed in reprogramming studies due to their abundance and accessibility. However, they are also the key drivers in the progression of fibrosis, a pathological...
Reprogramming of human fibroblasts using Sendai virus and the …
We have reprogrammed cells from four different human fibroblast cultures using Sendai virus and the Cellartis DEF-CS 500 Culture System (Figure 1). First, human BJ fibroblasts were reprogrammed to …
Direct fibroblast reprogramming: an emerging strategy for treating ...
Innovative methods for fibroblast reprogramming like small molecules, CRISPRa, modified mRNA, and the challenges of cellular heterogeneity and senescence faced by in vivo direct reprogramming, are …
High-efficiency RNA-based reprogramming of human primary fibroblasts
Feb 21, 2018 · Here, the authors show highly efficient reprogramming of human primary fibroblasts to iPSCs via the synergistic activity of synthetic modified mRNAs, mature miRNA mimics, and …
Fibroblast Reprogramming in Cardiac Repair - PMC
This review will discuss recent progress of fibroblast reprogramming in cardiac repair, including different types and states of reprogrammed fibroblasts, optimization of reprogramming protocols, in vitro …
Engineering fibroblast with reprogramming and spheronization for …
Aug 1, 2025 · Here, we present a method to engineer fibroblasts through small molecule reprogramming and spheronization, achieving functional osteoblastic materials across all age groups.
Dissecting the molecular trajectory of fibroblast reprogramming to ...
Aug 2, 2023 · Previously, we had already successfully established a technical platform for inducing fibroblast reprogramming to chemically induced mammary epithelial cells (CiMECs) by small …
Fibroblast Reprogramming in Cardiac Repair - JACC: Basic to ...
Sep 21, 2023 · This review will discuss recent progress of fibroblast reprogramming in cardiac repair, including different types and states of reprogrammed fibroblasts, optimization of reprogramming …
Direct reprogramming of mouse fibroblasts into self-renewable
Jun 23, 2025 · Here, we report the direct reprogramming of mouse tail tips and embryonic fibroblasts into induced pulmonary alveolar epithelial-like cells (iPULs) using four transcription factor-coding …
Fibroblast rejuvenation by mechanical reprogramming and ...
May 12, 2020 · Over the course of the aging process, fibroblasts lose contractility, leading to reduced connective-tissue stiffness. A promising therapeutic avenue for functional rejuvenation of connective …
Reprogramming - Wikipedia
Reprogramming can also be induced artificially through the introduction of exogenous factors, usually transcription factors. In this context, it often refers to the creation of induced pluripotent stem cells …
Direct fibroblast reprogramming: an emerging strategy for treating ...
Innovative methods for fibroblast reprogramming like small molecules, CRISPRa, modified mRNA, and the challenges of cellular heterogeneity and senescence faced by in vivo direct reprogramming, are …
Competing dynamic gene regulatory networks involved in fibroblast ...
May 13, 2025 · Investigating the gene regulatory networks involved during reprogramming would help generate functional cells in adequate numbers. To address this, we developed an inducible system …
Cancer-associated fibroblasts in the tumor microenvironment ...
Dec 18, 2025 · The tumor microenvironment (TME) is increasingly recognized as a critical modulator of the initiation, progression, metastasis, and therapeutic resistance of various cancers. Cancer …
Direct reprogramming of human fibroblasts into insulin ... - Nature
Mar 24, 2023 · This work provides proof-of-concept of the capacity to make insulin-producing cells from human fibroblasts via transcription factor-mediated direct reprogramming.
Direct Reprogramming of Fibroblasts to Osteoblasts: Techniques and ...
Dec 30, 2023 · Direct reprogramming (DR) is an emerging technique that can be applied to convert fibroblasts into osteoblast-like cells, promoting bone formation and regeneration.
Induced Pluripotent Stem Cell Reprogramming Protocols
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Reprogramming fibroblast into human iBlastoids - Nature
Apr 17, 2024 · Here we describe a step-by-step protocol for the generation of induced blastoids (iBlastoids), an in vitro integrated model of the human blastocyst derived via somatic reprogramming.
Fibroblast Reprogramming in Cardiac Repair - ScienceDirect
Jan 1, 2024 · This review will discuss recent progress of fibroblast reprogramming in cardiac repair, including different types and states of reprogrammed fibroblasts, optimization of reprogramming …