Essential cellular processes involving the actin cytoskeleton are regulated by auxiliary proteins that can sense the nucleotide state of actin. Here we report cryo-EM structures for ADP-bound and ...
Nucleotide exchange from ADP to ATP is crucially important for both the regulation and motility of the molecular motor kinesin 1,2,3,4,5. At least three key events occur during this step. First, this ...
Researchers used a novel phosphate-activation reagent, 2-MeImIm-Cl, in an improved coupling reaction to increase the yield of ADP- or ATP-containing molecules. Unlike traditional reactions, the ...
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